Journal: Veterinary research

Article Title: Potential of recombinant inorganic pyrophosphatase antigen as a new vaccine candidate against Baylisascaris schroederi in mice.

doi: 10.1186/1297-9716-44-90

Figure Lengend Snippet: Figure 7 Serum IgG and IgG subclass (IgG1 and IgG2a) responses induced by rBsc-PYP-1 in mice. Mice were tested for serum IgG and its subclasses before and after challenge with B. schroederi infective embryonated eggs either at 5 wpv (trial II) or at 6 wpv (trial I). Mice were subcutaneously vaccinated three times with rBsc-PYP-1 mixed with FCA in PBS at 14-day intervals, and serum samples were collected from the tail vein after each vaccination and at 0 (challenge or 6 wpv), 1, 3, 5, 6 and 7 wpc. Endpoint titers of anti-rBsc-PYP-1 IgG (A) and IgG-subclass (B) antibodies from immunized and control mice were determined by ELISA. Values are expressed as the mean ± SD for each group of six mice. Vaccination times (V1, V2, and V3) and challenge (C) are marked with black arrows, and asterisks in A and B indicate statistically significant differences between the rBsc- PYP-1/FCA/PBS vaccination group and two control groups (PBS plus FCA or PBS alone) (P < 0.001). Error bars represent SD.

Article Snippet: Affinity purified goat anti-mouse IgM, IgG, IgG1 and IgG2a (Bethyl Laboratories) were used as standards.

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: Journal of Veterinary Internal Medicine

Article Title: Investigation of the presence of specific neural antibodies in dogs with epilepsy or dyskinesia using murine and human assays

doi: 10.1111/jvim.16744

Figure Lengend Snippet: Canine anti‐IgLON5 antibodies. Immunofluorescence studies: Diluted serum (1:20) from a dog from the epilepsy/dyskinesia group (Golden Retriever, female, 3 years) is incubated with IgLON5‐transfected human embryonic kidney (HEK) cells. Binding of antibodies to cell surfaces is visualized by two different secondary anti‐canine antibodies coupled with immunofluorescence dyes and subsequently overlaid. The results demonstrate that there are canine anti‐IgLON5 IgG antibodies. Endpoint titration gave a titer of 1:40 (not shown). (A) anti‐canine IgG heavy and light chain (a sensitive secondary antibody), coupled with the red Alexa 594 dye. (B) overlay; yellow: double‐stainings. (C) anti‐canine IgG Fc fragment (the more specific secondary antibody), coupled with the green Alexa 488 dye. Bar: 25 μm.

Article Snippet: Secondary AB was a polyclonal rabbit anti‐dog immunoglobulin‐G (IgG) AB directed against canine IgG heavy and light chains (catalog no. 304‐065‐003; Jackson ImmunoResearch/Dianova) conjugated with red Alexa Fluor 594 used at a dilution of 1:100 and incubated for 30 minutes at room temperature; nuclei were counterstained with Hoechst 33342, 1:10 000.

Techniques: Immunofluorescence, Incubation, Transfection, Binding Assay, Titration

Journal: Journal of Translational Medicine

Article Title: TSLP promoting B cell proliferation and polarizing follicular helper T cell as a therapeutic target in IgG4-related disease

doi: 10.1186/s12967-022-03606-1

Figure Lengend Snippet: The expression of TSLP and its receptors in IgG4-RD. A Plasma levels of TSLP in HC, treatment naïve IgG4-RD patients and IgG4-RD patients with disease remission measured by Elisa. B Representative immunohistochemical staining of TSLP in LG from patients with SS (left) and SMG from patients with IgG4-RD (right). C The expression levels of TSLP in SMG estimated by semiquantitative analysis. D Trible staining for CD11c (green), CD4 (green), CD19 (green), CRLF2 (TSLPR, red) and DAPI (blue) in SMG from representative patients with IgG4-RD. E Percentages of TSLPR and IL-7Ra in CD4+, CD8+, CD19+, and Lin-CD11c+HLA-DR+ cells respectively. F Correlations between serum levels of IgG, IgE, IgG1, IgG4, RI and plasma level of TSLP. HC healthy controls, IgG4-RD IgG4-related disease, LG labial gland, SMG submandibular gland, SS primary Sjogren’s syndrome, RI responder index. *p < 0.05; ***p < 0.001; ns = not significant

Article Snippet: Antibodies in B cell culture supernatant was quantified by IgG Elisa kit (CUSABIO), IgG4 Elisa kit (CUSABIO) and IgE Elisa kit (Bethyl Laboratories).

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining

Journal: Journal of Translational Medicine

Article Title: TSLP promoting B cell proliferation and polarizing follicular helper T cell as a therapeutic target in IgG4-related disease

doi: 10.1186/s12967-022-03606-1

Figure Lengend Snippet: The effect of TSLP on B cells in IgG4-RD. CD19+ B cells from IgG4-RD patients were stimulated with IL-4, anti-IgM, CD40L, with or without TSLP. Proliferation quantified by Ki-67 ( A , B ), apoptosis measured by 7-AAD and Annexin-V ( C , D ), and B cell subsets ( E , F ) were assessed on 5 days. Supernatant IgG and IgG4 levels on 7 days were quantified by ELISA ( G ). *p < 0.05

Article Snippet: Antibodies in B cell culture supernatant was quantified by IgG Elisa kit (CUSABIO), IgG4 Elisa kit (CUSABIO) and IgE Elisa kit (Bethyl Laboratories).

Techniques: Enzyme-linked Immunosorbent Assay